Dye-fluorescence Led-spec: a Battery-operated, On-chip, Wavelength-tunable Optical Source for Detection of Biochemicals
نویسندگان
چکیده
ABSTRACT This paper reports a wavelength-tunable microfluidic optical source for the fluorescence of dye-labeled biochemicals. A pulsed micro-arc is struck between a metal anode and a microfluidic cathode filled with an aqueous solution of BaCl2. Atomic transitions from the Ba atoms, which are sputtered into the glow region, provide strong emissions at 493 nm and 454 nm wavelengths. Undesirable wavelengths are blocked out by an integrated optical filter. The emission is used to fluoresce calf thymus DNA tagged with SYBRTM green dye, achieving brightness levels comparable to commercially-available macro-scale apparatus. This method can be easily adapted for other fluorophores.
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